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C is a cosmogenic radionuclide that is formed continuously but not quite at a constant rate by the reaction of thermal neutrons with atmospheric nitrogen: It was the work of Willard Libby and his co-workers at the Institute for Nuclear Studies and Department of Chemistry at the University of Chicago that led to the development of the radiocarbon dating technique ( is taken up by green plants in the terrestrial biosphere and converted to carbohydrates by the process of photosynthesis.

Subsequent consumption of plants by animals (and animals by other animals) transfers the C enrichment between the well mixed atmosphere and the terrestrial flora and fauna that atmospheric carbon supports, provided that due corrections are made for the degree of isotopic fractionation that takes place during the initial uptake and subsequent metabolic fixation of atmospheric carbon by the primary producers (plant life) and thereafter, any isotopic fractionation that occurs during subsequent transport through the food chain.

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